Based on the forward group and reverse group data provided, what is the expected result of the antibody screen?

In evaluating the expected result of the antibody screen based on the forward and reverse grouping data, a positive outcome with all screening cells at room temperature (RT) and a negative autocontrol is indicative of the presence of antibodies in the plasma that are reacting with the antigens on the screen cells.

An antibody screen is designed to detect unexpected antibodies in the recipient's serum by using screening cells that represent the common blood group antigens. When the results show that all screen cells are reactive at RT and the autocontrol is negative, it suggests that the patient has developed antibodies against antigens present on the screening cells, but not against their own red blood cells. This pattern typically indicates the presence of non-autoantibodies (antibodies to foreign antigens) and supports the hypothesis that the patient has been sensitized to a specific antigen through prior transfusion, pregnancy, or other exposures.

This scenario suggests an immune response that is targeted and specific, which aligns with the reaction seen in the antibody screen. A negative autocontrol demonstrates that the patient's own red cells are not agglutinating with their serum, thereby ruling out the possibility of an autoantibody situation that would cause a false positive.

Understanding this helps reinforce the significance of the antibody screen in