To prepare a suitable anti-D reagent from a group O, Cde/Cde donor's serum containing anti-D, which cells would be suitable for the adsorption?

To prepare an effective anti-D reagent, it is essential to choose cells for adsorption that will effectively absorb the anti-D antibodies present in the serum from the group O, Cde/cde donor. The successful absorption depends on the Rh phenotype of the red blood cells used during the process.

Choosing group A1B, cde/cde cells is appropriate because these cells have the D antigen (as they are Rh-positive), which means they will interact with and effectively adsorb the anti-D antibodies from the serum. The presence of the D antigen on the red blood cells allows for the maximum interaction between the anti-D antibodies and the cells—leading to a purification of the anti-D reagent by eliminating nonspecific antibodies.

In contrast, options that include cells that are Rh-negative or do not have the D antigen would not sufficiently adsorb the anti-D antibodies in the serum. Group O, Cde/cde and Group O, cde/cde cells lack the D antigen and therefore would not be effective for this purpose. Group A2B, CDe/cde cells present a similar problem as they may have some D antigen, but not to the same level as a fully Rh-positive type such as A1B, limiting their effectiveness for anti-D adsorption