Which of the following methods is most effective for the elution of warm autoantibodies from RBCs?

The most effective method for the elution of warm autoantibodies from red blood cells (RBCs) is a change in pH. This method takes advantage of the decreased binding of antibodies as the pH of the solution is altered, typically by using an acidic environment. The change in pH can disrupt the interaction between the warm autoantibodies and the RBCs, allowing the antibodies to be released for further analysis.

This approach is particularly important in blood banking and transfusion medicine, as it allows for the identification of specific antibodies that may complicate transfusion or affect hemolytic disease of the newborn. In contrast, other methods such as 10% sucrose, LISS (low ionic strength solution), or distilled water may not be as effective or specific in displacing warm autoantibodies from RBCs.

10% sucrose is often used to stabilize cells during certain tests but is not effective in eluting antibodies. LISS can enhance antibody-antigen reactions in serological testing but does not generally promote the elution of antibodies from RBCs. Distilled water can cause lysis of RBCs, which may release some cellular components, but it is not a tailored approach for antibody elution and can lead